廖魯劍 博士 功能蛋白質組學

腦功能基因組學教育部重點實驗室PI
生命科學學院 教授
辦公電話：021-24207044
Email: ljliao@bio.ecnu.edu.cn

研究方向
以功能性蛋白組學為主要研究手段，進行神經系統疾病的蛋白質表達異常及其信號通路研究。基于大分子質譜學方法能對及其復雜的生物樣品進行大規模的蛋白質表達分析，實現蛋白質的翻譯后修飾檢測。同時應用大分子質譜學技術研究生物大分子的相互作用及在其他系統的蛋白質差異表達。這些重要的分析手段應用到重大疾病能發掘潛在的疾病發生發展的機制，亦能為臨床早期診斷提供依據。

個人簡歷
1988.9-1993.7          學士         湖南醫科大學，醫學檢驗
1995.9-1998.7          碩士         華西醫科大學，生物化學
1998.8-2002.12        博士         鮑林格林州立大學，生命科學，美國俄亥俄州
2003.2 - 2004.12    博士后  美國艾默蕾大學
2005.1 - 2007.3        博士后  美國斯特里普斯研究所
2007.4 - 2012.7        Staff Scientist 美國斯特里普斯研究所
2012.8 - 2013.7        中科院上海生命科學院生物化學與細胞生物學研究所
2013.8 – 至今         華東師范大學生命科學院

學術任職/人才獲獎
上海市“浦江人才”

代表性成果
Note: * Co-first author, # corresponding author.

1. Tang B, Wang T, Wan H, Han L, Qin X, Zhang Y, Wang J, Yu C, Berton F, Francesconi W, Yates JR 3rd, Vanderklish PW, and Liao L#. Fmr1 deficiency promotes age-dependent alterations in the cortical synaptic proteome. Proc Natl Acad Sci U S A. 2015; doi:10.1073/pnas.1502258112
2. Qin X, Zheng C, Yates JR 3rd, Liao L#. Quantitative phosphoproteomic profiling of PINK1-deficient cells identifies phosphorylation changes in nuclear proteins. Mol Biosyst. 2014 10(7):1719-29.
3. Li K, Zhou T, Liao L, Yang Z, Wong C, Henn F, Malinow R, Yates JR, Hu H. βCaMKII in Lateral Habenula Mediates Core Symptoms of Depression.  Science 2013;341:1016-1020.
4. Sando R 3rd, Gounko N, Pieraut S, Liao L, Yates JR 3rd, and Maximov A. HDAC4 Governs Transcriptional Programs Essential for Synaptic Development and Function. Cell. 2012；151(4):821-34.
5. Banerjee S, Liao L, Russo R, Nakamura T, McKercher SR, Okamoto S, Haun F, Nikzad R, Zaidi R, Holland E, Eroshkin A, Yates JR 3rd, Lipton SA. Isobaric tagging-based quantification by mass spectrometry of differentially regulated proteins in synaptosomes of HIV/gp120 transgenic mice: Implications for HIV-associated neurodegeneration. Exp Neurol. 2012; 236(2):298-306
6. McClatchy DB, Liao L, Lee JH, Park SK, Yates JR 3rd. Dynamics of subcellular proteomes during brain development. J Proteome Res. 2012; 11(4):2467-79
7. Liao L, Sando RC, Farnum JB, Vanderklish PW, Maximov A, and Yates JR 3rd. 15N labeled brain enable quantification of proteome and phosphoproteome in neurons. J Proteome Res. 2012; 11(2):1341-53
8. Mcclatchy D*, Liao L*, Park SK, Xu T, Lu B, and Yates JR. Differential proteomic analysis of mammalian tissues using SILAM. Plos One. 2011; 6(1): e16039
9. Liao L*, McClatchy DB*, Yates JR. Primer: shotgun proteomics in neuroscience. (Review) Neuron. 2009; 63(1):12-26
10. Park SK*, Liao L*, Kim JY, and Yates JR. A computational approach to correct arginine-to-proline conversion in quantitative proteomics. Nature Methods. 2009; 6(3):184-185
11. Liao L, Park SK, Xu T, Vanderklish PW, and Yates JR. Quantitative proteomic analysis of primary neurons reveals diverse changes in synaptic protein content in fmr1 knockout mice. Proc Natl Acad Sci USA. 2008; 105(40):15281-6
12. Liao L*, McClatchy DB*, Park SK, Xu T, Lu B and Yates JR. Quantitative analysis of brain nuclear phosphoproteins identifies developmentally regulated phosphorylation events. J Proteome Res. 2008; 7(11):4743-55
13. Xia Q*, Liao L*, Cheng D, Duong D, Gearing M, Lah JJ, Levey AI, and Peng J. Identification of novel kinases and ubiquitin ligases associated with Lewy bodies by comparative proteomics. Frontiers in Bioscience. 2008; 13:3850-6
14. Choe E, Liao L, Zhou J, Cheng D, Duong D, Jin P, Tsai L, and Peng J. Neuronal morphogenesis is regulated by the interplay between cyclin-dependent kinase 5 and the ubiquitin ligase mind bomb 1. J Neurosci. 2007; 27(35):9503-12
15. McClatchy DB, Liao L, Park SK, Venable J, and Yates JR. Quantification of the synaptic proteome of the rat cerebellum during postnatal development. Genome Res. 2007; 17(9):1378-88
16. Liao L, Pilotte J, Xu T, Wong CL, Edelman GM, Vanderklish PW, and Yates JR. BDNF induces widespread changes in synaptic protein content and upregulates the translation machinery components: an analysis using high-throughput proteomics. J Proteome Res. 2007; 6(3):1059-71
17. Liao L, Cheng D, Wang J, Losik T, Duong D, Gearing M, Rees H, Lah JJ, Levey AI, Peng J. Proteomic characterization of postmortem amyloid plaques isolated by laser capture microdissection. J Biol Chem. 2004; 279(35): 37061-8